Our results suggest that cellular uptake of nanoparticles is sensitive to the way cells are positioned and sedimentation need to be considered when performing .All these studies of applications and toxicity rely on our ability to quantify the interactions between nanoparticles and cells, including their uptake by cells.The MA-Au NPs possessed a negatively charged surface, whose zeta potential was around −20 m V in serum-containing medium regardless of the length of ligands.Whereas the total amount of adsorbed serum proteins had no significant difference, the relative amounts of adhesion mediators especially vitronectin (Vn) depended on the alkyl length significantly.The combined P(BCA/OCA) nanoparticles had an intermediate degradation rate.The uptake of POCA and PBCA nanoparticles was much higher in RBE4 than in PC3 cells. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.Recently, much attention has been given to the problem of drug delivery through the cell-membrane in order to treat and manage several diseases.
Efficient drug delivery requires both high cellular uptake of the nanoparticles and release of the drug from the nanoparticles.
Here we use upright and inverted cell culture configurations to show that cellular uptake of gold nanoparticles depends on the sedimentation and diffusion velocities of the nanoparticles and is independent of size, shape, density, surface coating and initial concentration of the nanoparticles.
Generally more nanoparticles are taken up in the upright configuration than the inverted one and nanoparticles that sediment faster showed greater differences in uptake between the two configurations.
Endocytosis inhibition studies showed that both clathrin- and caveolin-mediated endocytosis were involved in PACA nanoparticle uptake, and that the former played a predominant role, particularly in PC3 cells.
In the present study, we used three different optical techniques to show that within a 24-hour period PBCA nanoparticles degraded significantly inside cells, releasing their payload into the cytosol, while POCA nanoparticles remained intact.